Figure 1.
Intrahepatic CD8+ T cells expressed high level of PD-1 in IFNAR−/− mice infected by Ad. Wild-type and IFNAR−/− mice were i.v. infected with Ad and euthanized at day 6. (a) Top: flow cytometric examination of CD8+ T in IHLs; bottom-left: intracellular IFN-γ in CD3+CD8+ gate; bottom-right: numbers of IFN-γ+CD8+ T cells. (b) Surface staining of PD-1, CTLA-4, LAG-3, CD44 and Annexin V on CD8+ T in IHLs. N=6–8 mice per group. **P<0.01. Ad, adenovirus; IFN, interferon; IHL, intrahepatic lymphocyte; PD-1, program death 1.