Figure 5.

IL-7 suppressed PD-1 expression on CD8⁺ T cells. For a and b, CD4-depleted splenocytes from naive C57BL/6 mice were stimulated with anti-CD3 in the presence of IFN-β or IL-7. (a) CFSE dilution of CD8 T cells. (b) The flow cytometric histogram and MFI of PD-1 expression on CD8⁺ T cells. The experiments were repeated at least 3 times with a similar pattern. For c and d, wild-type and IFNAR^−/− mice were i.v. infected with Ad and euthanized at day 6. (c) IL-7 and IL-15 mRNA expression in the liver, compared with naïve control, respectively. (d) PD-1 expression (left: flow cytometric histogram; right: MFI) on intrahepatic CD8⁺ T cells from anti-IL-7Rα mAb-treated C57BL/6 mice infected with Ad. The cumulative statistical results are shown. N=5 to 8 mice per group. **P<0.01. Ad, adenovirus; IFN, interferon; mAb, monoclonal antibody; MFI, mean fluorescence intensity; PD-1, program death 1.