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. 2015 Mar 16;17(8):1205–1216. doi: 10.1111/cmi.12430

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© 2015 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 6 — Trypsin sensitivity of PfEMP1 in FCR3^Pfvap¹^Δ parasites. Synchronized FCR3 wild type and FCR3^Pfvap¹^Δ parasites (30–35 h) were selected by gel floatation prior to trypsin. Parasites were treated with either 0, 10 or 100 μg ml⁻¹ trypsin. The Triton X-100 insoluble/SDS soluble protein fraction was collected and separated by SDS-PAGE. A guinea pig anti-ATS antibody was used to detect PfEMP1. Treatment with trypsin results in the detection of an additional band (arrow) that represents the trypsin-resistant intracellular ATS domain. The membrane was then probed with the Hsp70 monoclonal antibody MAb1C11 as a loading control (bottom lane).