Skip to main content
. 2014 May 26;12(1):96–106. doi: 10.1038/cmi.2014.34

Figure 1.

Figure 1

Purification and analysis of purified rIL-22 and rGroEL. (a) (i) SDS–PAGE of rIL-22. Lane 1: purified recombinant IL-22; lane 2: commercial IL-22 (positive control). (ii) Western blot of rIL-22. (b) (i) SDS–PAGE of rGroEL. Lane M: molecular weight marker. Lanes 1 and 2 show purified rGroEL protein. (ii) Western blot of rGroEL. (c) rIL-22 promotes murine lymphocyte proliferation. Cell proliferation was evaluated by MTT assay. Significant proliferation was induced by rIL-22 compared to the control (*P<0.001 vs. control). The data are presented as the mean of three independent experiments. SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.