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. 2014 May 26;12(1):96–106. doi: 10.1038/cmi.2014.34

Figure 4.

Figure 4

Effect of codelivery of rGroEL antigen and IL-22 on cytokine levels. Mice were immunized with rGroEL or rGroEL+rIL-22. One week after the last immunization, splenocytes/peritoneal macrophages isolated from mice were cultured (1×106 cells/well) in the absence (unstimulated) or presence (stimulated) of rGroEL for 72 h. Cytokines were estimated in culture supernatants collected from control and immunized groups by ELISA: (a) IFN-γ, (b) IL-1β, (c) IL-6, (d) IL-4 and (e) IL-10. The data are presented as the mean concentration (pg/ml)±s.d. and are representative of three independent experiments and compared by one-way ANOVA. *P<0.01 vs. control, #P<0.01 vs. rGroEL. ANOVA, analysis of variance.