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. 2014 Sep 8;12(1):113–115. doi: 10.1038/cmi.2014.67

Figure 1.

Figure 1

Inflammatory responsive miRNA identified using a computational approach and its stem-loop RT-PCR expression analysis in rat hepatocytes with LPS induction. (a) Hairpin structure of predicted Rattus norvegicus miRNA rno-miR-24; (b) hairpin structure of predicted Rattus norvegicus miRNA rno-miR-466i-5p; (c) details of predicted Rattus norvegicus miRNA; (d) RT-PCR expression analysis of pro-inflammatory gene (COX2 and iNOS) expression fold ratio with time dependence; (e) stem-loop RT-PCR expression analysis of miRNA (rno-miR-24 and rno-miR-466i-5p) expression fold ratio with time dependence. LPS, lipopolysaccharide; microRNA, miRNA; RT-PCR, reverse transcription polymerase chain reaction.