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. 2015 Nov 18;56(12):7496–7515. doi: 10.1167/iovs.15-17616

Figure 4.

Figure 4

CRISPR/Cas9 targeted mutation of neurod phenocopies NeuroD knockdown and similarly affects Notch signaling. (A) EdU and Zpr-1 (cones) labeling in retinas of uninjected and WT injected (T7 endonuclease [EN]-negative) embryos, compared to retinas of 3 embryos positive for insertions and/or deletions in the neurod gene (T7 endonuclease [EN]-positive). Zpr-3 labeling (rods) was affected identically (data not shown). As a relative comparison of photoreceptor progenitors still in the cell cycle in the central retina, arrowheads indicate the central-most EdU+ cells in the ONL. (B) In situ hybridization showing retinal expression of notch1a, dla, dld, ascl1a, and her4 in embryos with neurod indels compared to uninjected controls. Arrowheads indicate zones of expression; circled regions highlight expression differences in and around the developing ONL and INL adjacent to the dorsal CMZ. Scale bars: 50 μm.