. Author manuscript; available in PMC: 2016 Dec 1.

Published in final edited form as: Bioorg Med Chem Lett. 2015 Oct 23;25(23):5514–5519. doi: 10.1016/j.bmcl.2015.10.066

Table 4.

IC₅₀, water solubility and microsomal stability of selected compounds.

#	IC₅₀ nM^a	Solubility^b (μM)	cLogP^c	Microsomal stability (%)^d NADPH
#	IC₅₀ nM^a	Solubility^b (μM)	cLogP^c	−	+
3a	15.6 ± 0.7	75 < S < 100	4.24	97 ± 4	0.4 ± 0.2
4a	28.8 ± 4.5	125 < S < 150	4.50	115 ± 11	0.4 ± 0.2
3b	12.5 ± 1.8	100< S < 125	3.85	87 ± 6	0.8 ± 0.2
4b	18.0 ± 2.9	25 < S < 50	3.89	102 ± 11	0.4 ± 0.3
3c	4.9 ± 0.1	75 < S < 100	2.59	91 ± 8	0.4 ± 0.1
4c	4.8 ± 0.6	75 < S < 100	2.63	95 ± 1	0.3 ± 0.1
3d	9.9 ± 2.9	450 < S < 475	2.36	72 ± 4	2.9 ± 0.5
4d	111.1 ± 10.3	200 < S < 225	2.62	83 ± 3	0.1 ± 0.1
3g	13.7 ± 3.0	75 < S < 100	3.91	97 ± 10	0.3 ± 0.1
4g	14.2 ± 2.5	75 < S < 100	4.17	92 ± 6	0.4 ± 0.1

As determined via a kinetic fluorescent assay.^{34, 35} Results are average ± standard deviation of three separate measurement.

Solubility was measured in sodium phosphate buffer (pH 7.4, 0.1 M) containing 1% of DMSO.

Calculated using ChemBioDraw Ultra v12.0 (PerkinElmer, Waltham, MA).

Percent of compound (1 μM) remaining after 30 minutes incubation with human liver microsomes (1 mg/mL) at 37°C with or without NADPH generating system. Results are the average of triplicates ± standard deviation.