Skip to main content
. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: ACS Chem Biol. 2015 Feb 24;10(5):1176–1180. doi: 10.1021/cb5009684

Figure 2.

Figure 2

Generation of the H4Kc20me2 mark, a chemical analog of H4K20me2. (A) Amino acid sequence of the H4(14–25) K20C peptide, with an extra tyrosine introduced to the C-terminus for spectrophotometric measurement. (B) Schematic depicting the generation of the H4Kc20me2 modification.24 (C) Mass spectra of the H4(14–25) K20C and H4(14–25)Kc20me2 peptides. The peaks indicated by the asterisks arise from the isotope effects. (D) ITC analysis of the bindings of wild type hORC1BAH and its R105Q mutant to the histone H4(14–25)Kc20me2 peptide.