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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Prostate. 2015 Mar 1;75(9):907–916. doi: 10.1002/pros.22974

Figure 2. Knockdown of Slug upregulates PTEN expression.

Figure 2

(A) Western-blot analysis of PTEN expression in prostate cancer cell lines stably expressing Slug shRNA. 22RV1 (left panel) and DU145 (right panel) cells were infected with lentiviruses expressing SLUG-specific shRNAs and control shRNA, and then selected with puromycin and expanded. Total protein was extracted from respective cell lines and analyzed by Western-blot analysis using anti-SLUG, anti-pAKT, anti-PTEN and anti-GAPDH (loading control) antibodies, respectively.

(B) qPCR analysis of PTEN transcripts in stable cell lines expressing SLUG-specific shRNAs and control shRNA. ** p < 0.01.