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. 2015 Nov 20;10(11):e0143275. doi: 10.1371/journal.pone.0143275

Fig 1. CHD8 depletion by RNAi is detrimental to B-ALL cells in vitro and in vivo.

Fig 1

(A) Schematic of the GFP growth competition assay. An increase in the percentage of GFP+ cells indicates positive selection conferred by shRNA expression, while a decrease indicates negative selection. (B) Western blot showing decreased CHD8 expression in B-ALL cells conferred by both shRNAs (4 days following retroviral infection). (C, D) Results of in vitro (C) and in vivo (D) growth competition assays. Shown are averages ± SEM of four independent experiments (C) and averages ± SD of 5 mice (D). Fold changes of in vitro assays were calculated from day 2 to day 10 after retroviral infection and normalized to an empty vector control. Fold changes of in vivo assays were calculated from day 2 after retroviral infection (day of injection) to morbidity (approximately 10 days later). In vivo assay shRNA results were compared with empty vector control in each respective tissue. (E) Kaplan-Meier curve showing survival of mice injected with B-ALL cells transduced with the indicated constructs. n = 15 per cohort. (F) Graph showing GFP expression of cells harvested from the indicated mice upon morbidity. **P < 0.005, ***P = 0.002, ****P < 0.0001