Table 1. Parameters (τr) describing the mobility of the spin label of αS bound to natural membranes from simulations of cw-EPR spectra.
condition | αS spin label positions | fast component | slow component | immobile component | |||
---|---|---|---|---|---|---|---|
τ r (ns) | contribution (%) | τ r (ns) | contribution (%) | τ r (ns) | contribution (%) | ||
buffer | αS56 | 0.45± 0.02 | 100 | na | na | na | na |
αS69 | 0.31±0.02 | 100 | na | na | na | na | |
IMM | αS56 | 0.45 | 2.0±0.5 | 2.88±0.13 | 90 ±1.5 | >50.0 | 8.0 |
αS69 | 0.31 | 2.0±0.5 | 2.23±0.11 | 98 ±1.0 | na | na | |
NPM | αS56 | 0.45 | 2.0±0.5 | 2.95±0.14 | 88±1.5 | >50.0 | 10.0 |
αS69 | 0.31 | 2.2±0.3 | 1.99±0.13 | 98±0.3 | na | na |
na: not contributing in the simulation. For error determination, see Materials and Methods.