Fig 3. Optimization of 17β Estradiol and Progesterone dose supplement for uterine fibroid xenograft growth.

Ovariectomized SCID outbred mice were either not treated or supplemented with Progesterone pellets as indicated, and standard 17β Estradiol pellets (0.05mg/90d release, panel A); or 17β Estradiol pellets as indicated, and standard Progesterone pellets (25mg/60d release, panel B). Small pieces of fibroid tissue where grafted at an average weight indicated with the dotted line. For this experiment, 4 to 5 mice per donor with eight grafts each where used per treatment group. Mice were sacrificed after 60d, and grafts were weighted. Symbols indicate individual graft weights, together with group means ± s.d. Significance of difference between treatment and control groups was evaluated a two-sided t-test with Dunnett´s correction for multiple testing.