Fig 2. Tenfold magnification of Masson’s trichome staining of acute, 3 weeks, and 3 months follow-up histology.

Acute (A), 3 weeks (B), and 3 months (C) histology results (10x magnification) showing the border zone of lesions in treated vessels. Sections were stained with MST staining. The arrows indicate the border zone. (A) Border zone of lesion immediately after denervation. The border zone is characterized by a red to pink color transition. At the border zone cell depletion is present (white holes). (B) Border zone of lesion three weeks after denervation. The border zone is characterized by a red to light green color transition. At the site of de border zone inflammatory cells are present (tiny dark blue/black spots). (C) Border zone of lesion 3 months after denervation. The border zone is characterized by blue to red color transition. At the border zone increased collagen deposition is present (blue fibers) and the collagen fibers are intertwined the adjacent healthy muscle tissue of the media (red).