Table 1. Detailed primers and conditions used for qRT-PCR assays.
| Cytokine/Housekeeping Gene | Primer Sequence a | Annealing Temp (°C) | T m (°C) b | Amplicon Length (bp) a | Nucleotide Sequence Accession Numbers |
|---|---|---|---|---|---|
| TNF-α | F: AACGGCATGTCTCTCAA | 60 | 50.4 | 278 | AF046215 |
| R: AGTCGGTCACCTTTCT | 49.2 | ||||
| IFN-γ | F: GACAACCAGGCCATCC | 60 | 54.3 | 226 | AF034482 |
| R: CAAAACAGCACCGACT | 49.2 | ||||
| TGF-β | F: ACGGAGAAGAACTGCT | 60 | 42.9 | 245 | AF046214 |
| R: ACGTAGTACACGATGGG | 52.8 | ||||
| IL-1α | F: AGTTCGTCCTGAATGATTCC | 61 | 58.4 | 202 | AB028235 |
| R: TGGTCTTCACCCTGAGC | 59.6 | ||||
| β-Actin | F: TCTACAACGAGCTGCG | 60 | 51.7 | 357 | AJ312092 |
| R: CAATTTCCCTCTCGGC | 51.7 |
aVernel-Pauillac and Merien, 2006; Vernel-Pauillac and Goarant, 2010.
bMelting temperature.