Table 1. Primers and product size of genes used for validation using quantitative real-time polymerase chain reaction.
| Gene symbol a | GenBank accession number | Forward (F) primers 5'-3' | Product size (base pairs) |
|---|---|---|---|
| Reverse (R) primers 5'-3' | |||
| HSP25 | NM_001010842 | F: CCGTCTTCTGCTGAGAGGAGTG | 117 |
| R: ACCGTTGTTCCGTCCCATCAC | |||
| IL6 | NM_204628 | F: AGCAAAACACCTGTTACATTTCT | 96 |
| R: AGTCTGGCTGCTGGACATTT | |||
| VTG2 | NM_001031276 | F: CAGCCTAACTGACAAACAGATGAAG | 100 |
| R: GCATTCCTCATTCTCACATGAACAC | |||
| MMP13 | AF070478 | F: TTGGTGCTAAGTATAGATGAATGCC | 131 |
| R: TGTAGGTAGTCAGTGCTTGTTCG | |||
| PTRF | NM_001001471 | F: CCCTGCCTGCTAGGACAAG | 149 |
| R: AGGTCTGGGCTCTGGAAGG | |||
| COL2A1 | NM_204426 | F: CACTGAACGGATGGCACGAC | 137 |
| R: CCTCCACCCGCCCTACG | |||
| DDR2 | CR387623 | F: TGCGGACGGGAGGAACTG | 103 |
| R: AGCAATAGGGTACTGCGAATGG | |||
| KLF2 | XM_418264 | F:CGCCGAGGATTGGACACAG | 139 |
| R: CACGGAGTTCACCCTTCACAG | |||
| GAPDH | NM_204305 | F: CATCACAGCCACACAGAAGA | 122 |
| R: TGACTTTCCCCACAGCCTTA |
a Abbreviations: HSP25, heat shock protein 25; IL6, interleukin 6; VTG2, vitellogenin 2; MMP13, metalloproteinase 13; PTRF, polymerase I and transcript release factor; COL2A1, type II alpha 1 collagen; DDR2, discoidin domain receptor tyrosine kinase 2; KLF2, Kruppel-like factor 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.