Table 2. Multiples of changes of significantly differentially expressed genes in small yellow follicles of broiler-type B strain Taiwan country chickens after acute heat stress determined using microarray and quantitative reverse transcription polymerase chain reaction analyses.
| Fold change* | Gene a | |||||||
|---|---|---|---|---|---|---|---|---|
| HSP25 | IL6 | VTG2 | MMP13 | PTRF | COL2A1 | DDR2 | KLF2 | |
| H2R0/CTL | ||||||||
| M | 34.42 | 2.54 | 8.88 | 0.76 | 2.18 | 0.68 | 0.41 | 0.32 |
| Q | 54.40 | 1.93 | 3.37 | 0.96 | 0.91 | 0.81 | 1.09 | 0.27 |
| H2R2/CTL | ||||||||
| M | 38.20 | 2.11 | 1.21 | 3.21 | 2.08 | 0.98 | 0.82 | 0.95 |
| Q | 36.39 | 3.05 | 1.01 | 3.40 | 0.84 | 0.48 | 3.98 | 0.32 |
| H2R6/CTL | ||||||||
| M | 10.46 | 0.96 | 1.75 | 0.73 | 2.03 | 0.43 | 0.41 | 0.43 |
| Q | 8.40 | 1.82 | 1.65 | 0.82 | 0.74 | 0.53 | 0.94 | 0.34 |
* Multiples of changes of two-fold or higher increase or decrease were defined as different (p < 0.05). The fold expression or repression of the target gene were normalized using glyceraldehyde-3-phosphate dehydrogenase as an internal control gene.
a Abbreviations: HSP25, heat shock protein 25; IL6, interleukin 6; VTG2, vitellogenin 2; MMP13, metalloproteinase 13; PTRF, polymerase I and transcript release factor; COL2A1, type II alpha 1 collagen; DDR2, discoidin domain receptor tyrosine kinase 2; KLF2, Kruppel-like factor 2.