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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Biochem J. 2014 Mar 1;458(2):335–341. doi: 10.1042/BJ20130818

Figure 5. Activation of CREB is involved in NRG-1β-induced cardiomyogenesis of mESC differentiation.

Figure 5

NRG-1β treatment from day 0 to 2 significantly induced active phospho-CREB (p-CREB) and transient knockdown of ErbB3 with siRNA dramatically attenuated active phospho-CREB in the presence of NRG-1β (a). CREB siRNA effectively reduced CREB expression and levels of phospho-CREB during the initial 48 h of mESC treatment with 50 ng/ml NRG-1β (b) and prevented cardiomyogenesis as indicated by reduced Myh6 expression on day 10 (c). Myh6 RNA expression in the siRNA control (Ctr) samples was set to 1, and the relative RNA expression level was calculated by normalizing Myh6 RNA expression with its RNA expression in the siRNA control samples. The qPCR results were obtained from two independent experiments.