| H2O |
to 500 ml |
|
| 50x BME amino acid solution (Sigma B6766) |
50 ml |
5X |
| L-glutamate potassium salt (Sigma G1149) |
500 mg |
1.0 mg/ml |
| Glycine (Sigma G-7126) |
200 mg |
0.4 mg/ml |
| Sucrose (Sigma |
3.75 g |
7.5 mg/ml |
| Biotin (Sigma B4639) |
500 μl 10 mg/ml stock |
0.02 μg/ml |
| B12 (Sigma V6629) |
500 μl 14 mg/ml stock |
0.028 μg/ml |
| Choline chloride (Sigma C7527) |
37.5 mg |
75 μg/ml |
| Ca-pantothenate (Sigma C8731) |
3 mg |
6 μg/ml |
| Pyridoxin-HCl (Sigma P6280) |
1.5 mg |
3 μg/ml |
| Riboflavin (Sigma R-0508) |
1.2 mg |
2.4 μg/ml |
| Thiamine-HCl (Sigma T1270) |
0.75 mg |
1.5 μg/ml |
| Folic acid (Sigma F858) |
18 mg |
36 μg/ml |
| MnCl2.4 H2O (Sigma M3634) |
24 mg |
48 μg/ml |
| MgSO4.7 H2O (Sigma M-9397) |
123 mg |
0.246 mg/ml |
| K2HPO4 (P285-500 Fischer) |
303 mg |
0.606 mg/ml |
| KH2PO4 (P288-500 Fischer) |
303 mg |
0.606 mg/ml |
| NaCl (Sigma S7653) |
6.45 mg |
0.0129 mg/ml |
| CaCl2 (Sigma C-5080) |
6.45 mg |
0.0129 mg/ml |
| FeSO4.6 H2O (Sigma F8633) |
11.8 mg |
0.0129 mg/ml |
