Table 1.
Figures of merit used for tracking microarray scanner performance in this study
| Definition | |
|---|---|
| Column 25 signal intensitya | Mean signal intensity of 12 spots at a given concentration |
| Column 25 signal to noise (S/N) | Mean signal intensity of column 25 relative to the standard deviation of the 12 spots of column 25 |
| On-spot background | Median signal of spots in columns 5–10, all of which are below the LOD |
| Slope (sensitivity) | Signal intensity relative to concentration (measured as column number) |
| Limit of detection (LOD) | Measure of the smallest amount of dye (column number) that can be detected at given conditions |
a
Column 25 was chosen due to its position in the middle of the linear region.