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Instrument settings used for dye slidesa
| Lots A and B | Lot C (printed approximately nine months before use) | ||||
|---|---|---|---|---|---|
| Cy5 | Cy3 | Cy5 | Cy3 | ||
| Scanner R | Gain (V) | 725 | 500 | 800 | 540 |
| Laser power (%) | 33 | 33 | 33 | 33 | |
| Scanner S | Gain (V) | 550 | 500 | 600 | 500 |
| Laser power (%) | 20 | 5 | 20 | 5 | |
The instrument settings were chosen based on saturation of 4–6 columns, as recommended by the dye slide manufacturer. Settings were kept constant throughout the 5-week study.
