. Author manuscript; available in PMC: 2015 Nov 20.

Published in final edited form as: J Mol Biol. 2012 Feb 7;421(0):315–328. doi: 10.1016/j.jmb.2012.01.055

Table 2.

Experimental kinetic and thermodynamic parameters for Aβ40 and Aβ40 ^DPG variant fibril self-assembly.

	Kinetic effects				Thermodynamic effects

Peptide	t_1/2	k (h⁻¹)	lag time (h)	C_r (µM)	ΔG (kcal mol⁻¹) [kJ/mol]	ΔΔG (kcal mol⁻¹) [kJ/mol]
wild-type	91 ± 7	0.3 ± 0.1	83 ± 7	5.7 ± 2.3	−7.2 ± 0.2 [−30 ± 1]	-
^DPG-24,25	71 ± 8	0.2 ± 0.1	60 ± 7	51.7 ± 1.8	−5.8 ± 0.1 [−24 ± 1]	1.4 ± 0.1 [6 ± 1]
^DPG-25,26	< 10 h^a	-	-	9.3 ± 2.0	−6.9 ± 0.1 [−29 ± 1]	0.3 ± 0.1 [1 ± 1]
^DPG-26,27	< 20 ha	-	-	18.0 ± 1.7	−6.5 ± 0.1 [−27 ± 1]	0.7 ± 0.1 [3 ± 1]

Data could not be fit to a sigmoid equation because there was no observable lag phase; t_1/2 was approximated as the time at which ThT emission reaches half the maximal value.