Wild type (WT), β-arrestin2 knockout (β-arr2−/−) and β-arrestin2 heterozygous (β-arr2+/−) mice were fed 3.5% DSS in their drinking water for 6 days to induce colitis and euthanized on day-7. (a) Percentage body weight loss and (b) disease activity index observed over the course of the experiment. (c) Colon length, (d) thymus and (e) spleen weight recorded for control and colitic mice at day 7. N=14–28 mice per genotype. Data pooled from atleast three independent experiments. * p<0.05, **p<0.01, ***p<0.001 as compared to WT using 2-way ANOVA for weight loss and DAI and student’s t-test for colon length, spleen and thymus weight.