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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Nature. 2015 Oct 28;527(7578):371–374. doi: 10.1038/nature15758

Figure 2. Bipolar tail neuron precursors delaminate and migrate.

Figure 2

a, Embryos electroporated with Neurog b-line>unc-76∷eGFP. Upper: Bipolar tail neuron (BTN) precursor (arrowhead) extending a lamellipodium. Lower: BTN precursors (anterior, aBTN and posterior, pBTN) delaminating. Scale bar, 25 μm. b, Enlarged view of aBTN in lower panel of a. Upper: UNC-76∷eGFP. Middle: Phalloidin. Lower: merged, arrowhead: part of aBTN still in the epithelium. Scale bar, 10 μm. c, Embryo with paraxial mesoderm labeled by Mrf>unc-76∷eGFP (green), BTN labeled by Neurog b-line>unc-76∷mCherry (magenta) and phalloidin counterstain. Scale bar 25 μm. Right: Cross sectioned 3D image of same embryo. Only the right side of the embryo was transfected. d, 3D slice of embryo showing BTNs (arrowheads) outside neural tube (dotted outline). Only the right side of the embryo was transfected. e, Diagram of d, showing BTNs relative to other tail tissues: neural tube (NT), notochord (NC), myoblasts (M), and endoderm (EN). f, Time series of different embryos co-electroporated with Neurog b-line>unc-76∷VenusYFP (green) and Nodal b8.18>H2B∷mCherry (magenta). Right panels are enlarged views from left. Dashed lines indicate displacement from clonally-related epidermal cells (indicated by color-coded brackets). Scale bars, 25 μm.