Fig. 7.

a Phylogenetic relationship between Rab sequences of Leishmania and Leptomonas which is based on multiple sequence alignment (ClustalW algorithm in MEGALIGN with 1000 bootstraps). A dotted line on a phenogram indicates a negative branch length, a common result of averaging and Seed 111 indicates “Random Seed: This option makes bootstrapping consistent with the Clustal interface. The random seed is the value that initializes the pseudo-random number generator. Choosing the same seed causes the generator to produce exactly the same sequence of numbers for a given set of distances and hence the same result. Choosing a different seed value produces an alternative sequence of numbers which should introduce some variation in the result, but it should be similar. The highly conserved values show which parts of the tree should be given greater confidence”. PrePS analysis (PrePS- Prenylation Prediction Suite, http://mendel.imp.ac.at/sat/PrePS/index.html) between Rab 2A and Rab11B of Leishmania showed that b Rab11B lacks the cysteine residue at C terminus of CAAX signal sequences which is essential for prenylation. c Rab 2A has cysteine residue at C terminus of CAAX signal sequences