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. 2015 Jan 15;55(1):55–64. doi: 10.1111/cga.12099

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© 2014 The Authors. Congenital Anomalies published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Teratology Society.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Chimeric mouse generated by In utero hematopoietic cell transplantation. Lineage-depleted cells from bone marrow of ICR/B6-GFP were administered into the vitelline veins of fetuses, which were developed from transferred homozygous embryos in the surrogate mother (A). Chimerism was confirmed by flow cytometry. Representative flow analysis of green fluorescent protein (GFP)-positive cells in a chimeric MPSVII mouse (B-E). (B) Gate for live cells. (C) Mononuclear cell fraction (red circle) and granulocyte fraction (black circle). (D, E) Donor engraftment rates in mononuclear cell fraction (D) and in granulocyte fraction (E) (see Table 1).