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. 2015 Nov 21;16:27. doi: 10.1186/s12858-015-0056-3

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© Kurdyumov et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — SDS-PAGE of mlDL-Ds3. mlDL-Ds3 was isolated using metal chelate affinity chromatography. mlDL-Ds3 was heating 10 min at 95 °C with 2 % 2-mercaptoethanol. M—molecular weight marker (kDa). Electrophoresis was performed in 13.5 % acrylamide/bis gel. The gel was stained with Coomassie 250G