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. 2015 Nov 20;9:21. doi: 10.1186/s13036-015-0019-7

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© Wei and Smolke. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Reliability of cell cycle control systems over time. a Performance of the cell cycle controller for G0/1 (integrated p27-switch controller) over time. Cells harboring the G0/1 cell cycle controller (p27 switch th-A) or a negative control (p27 OFF control) were induced with 0 or 1 mM theophylline (theo) and the percentage of the cell population in G0/1 was measured via DNA staining and flow cytometry after creation of the cell lines (left of hash marks) and at 1 and 3 weeks after cell lines were frozen and revived (right of hash marks). b Performance of the cell cycle controller for G2/M (integrated CCNB1m-switch controller) over time. Cells harboring the G2/M cell cycle controller (CCNB1m switch th-A) or a negative control (CCNB1m OFF control) were induced with 0 or 1 mM theophylline and the percentage of the cell population in G2/M was measured via DNA staining and flow cytometry at 1, 2, 3 and 6 weeks after creation of the cell lines