Mice were treated with 2 mg tamoxifen i.p. daily for 3 days and observed for gross pathology.
(A) Samples of the ear were genotyped for Adar deletion following tamoxifen treatment.
(B) Surface body temperate readings for tamoxifen-treated mice from 20–120 hours after initial tamoxifen injection.
(C) Serum cytokine levels determined from mice at Day 3 or Day 8.
(D) Ex vivo dissection of the gastrointestinal tract at Day 8; Stomach (S), small intestine (SI), cecum (Ce) and large intestine (LI) are indicated.
(E) Representative hematoxylin and eosin-stained ileum sections of tamoxifen-treated mice of the indicated genotypes at Day 8. Original magnification is indicated in the bottom right.
(F) Histological scoring of inflammation in the gut at Day 8. SI=small intestine; CE=cecum; Prox=Proximal colon; Mid=Mid-colon. Mean inflammation score of Adarfl/flMavs−/− UBC:Ert2Cre+ intestines compared to either control is significant by one-way ANOVA, p=0.002.