Skip to main content
. Author manuscript; available in PMC: 2016 Nov 17.
Published in final edited form as: Immunity. 2015 Nov 17;43(5):1011–1021. doi: 10.1016/j.immuni.2015.10.016

Figure 5. FTY720 treatment preferentially blocks migration of Treg cells into neonatal skin.

Figure 5

FTY720 or saline was administered every 48 hours between postnatal D5 and D11 and skin, thymus and SDLNs were harvested on D13. (A) Flow cytometry and (B) percent Treg cells in skin on D13 vs. untreated D6 neonates. Gated on live CD45+CD3+CD4+ cells. (C) Absolute numbers skin of Treg cells and CD4+FoxP3neg (Teff) cells in skin on D13. (D) Absolute numbers of Treg cells in thymus and SDLNs on D13. Absolute numbers of (E) CD8+ T cells, (F) dermal γδ T cells, (G) dendritic epidermal T cells (DETC) and (H) CD45+CD3neg myeloid cells in skin on D13. Data is representative of two independent experiments with ≥3 mice per group.