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Osteocalcin expression in (a) collagen-spheroids-dentin, (b) collagen monolayer fibroblast-dentin, (c) PGA-spheroid-dentin, and (d) PGA-monolayer fibroblast-dentin cultures after 20 days. Original magnification ×40 (scale bar = 50 μm). Green arrows show nonspecific staining.
