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Effect of MPP+ on cell viability of undifferentiated and differentiated SH-SY5Y cells. Cells were differentiated in 10-μM retinoic acid (RA) for 3 days. Undifferentiated and RA-differentiated cells were plated at the same time and exposed to 125, 250, 500, 1000, and 2000 μM of MPP+ for 24 hours. Cell viability was examined using MTT assay. Data are expressed as fold of changes (FOC; mean ± SEM), relative to control (n = 3). ∗ P < 0.05; ∗∗ P < 0.01; and ∗∗∗ P < 0.001.
