Figure 5.
Effect of MPP+ on Bax and Bcl-2 mRNA expression in undifferentiated and differentiated SH-SY5Y cells. Cells were differentiated in 10-μM retinoic acid (RA) for 3 days. Undifferentiated (a–c) and differentiated cells (d–f) were exposed to 500 and 1000 μM of MPP+ for 24 hours, respectively. Expression of Bax mRNA (a and d), Bcl-2 mRNA (b and e), and Bax/Bcl-2 ratio (c and f) was analyzed with quantitative real-time RT-PCR, relatively compared to their respective untreated controls. The expression levels of the target gene were normalized to the expression level of β-actin. Data are expressed as mean ± SEM (n = 3). ∗∗ P < 0.01.