Table 4.
MIC of antibiotics after the association of the extract of Beilschmedia acuta at MIC/2 and MIC/5 against selected MDR bacteria
| Antibioticsa | Extract and concentration | Bacterial strainsb, MIC (μg/mL) of antibiotics in the absence and presence of the extract and FIC in parenthesis | PBSS (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AG102 | AG100ATET | EA27 | CM64 | KP55 | NAE16 | BM67 | PA01 | PA124 | ||||
| CIP | 0 | 4 | 64 | 4 | 64 | 4 | 128 | 32 | 16 | 32 | ||
| L | MIC/2 | 4 (1)I | 64 (1)I | 1 (0.25)S | 64 (1)I | 8 (2)A | 128 (1)I | 16 (0.50)S | 16 (1)I | 16(0.50)S | 3/9 (27.27 %) | |
| MIC/5 | 4 (1)I | 64 (1)I | 2 (0.50)S | 64 (1)I | 8 (2)A | 128 (1)I | 32 (1)I | 16 (1)I | 32(1)I | 1/9 (11.11 %) | ||
| B | MIC/2 | 4 (1)I | 32 (0.50)S | 1 (0.25)S | 64 (1)I | 4 (1)I | 128 (1)I | 16 (0.50)S | 16 (1)I | 32(1)I | 3/9 (27.27 %) | |
| MIC/5 | 4 (1)I | 64 (1)I | 2 (0.50)S | 64 (1)I | 4 (1)I | 128 (1)I | 32 (1)I | 16 (1)I | 32(1)I | 3/9 (27.27 %) | ||
| TET | 0 | 8 | 64 | 64 | 32 | 2 | 64 | 32 | 64 | 16 | ||
| L | MIC/2 | 2 (0.25)S | 32 (0.50)S | 32 (0.50)S | 8 (0.25)S | 2 (1)I | 32 (0.50)S | 32 (1)I | 16 (0.25)S | 8(0.50)S | 7/9 (77.78 %) | |
| MIC/5 | 4 (0.50)S | 64 (1)I | 64 (1)I | 16 (0.50)S | 2 (1)I | 64 (1)I | 32 (1)I | 64 (1)I | 8(0.50)S | 3/9 (27.27 %) | ||
| B | MIC/2 | 4 (0.50)S | 32 (0.50)S | 16 (0.25)S | 16 (0.50)S | 1 (0.50)S | 16 (0.25)S | 16 (0.50)S | 32 (0.50)S | 8(0.50)S | 9/9 (100 %) | |
| MIC/5 | 4 (0.50)S | 64 (1)I | 64 (1)I | 16 (0.50)S | 1 (0.50)S | 32 (0.50)S | 32 (1)I | 64 (1)I | 16(1)I | 4/9 (36.36 %) | ||
| KAN | 0 | - | 16 | 128 | 4 | 16 | 16 | 64 | 4 | 128 | ||
| L | MIC/2 | 8(˂0.03)S | 4 (0.25)S | 64 (0.50)S | 2 (0.50)S | 16 (1)I | 16 (1)I | 32 (0.50)S | 4 (1)I | 64(0.50)S | 6/9 (66.67 %) | |
| MIC/5 | 64 (˂0.25)S | 16 (1)I | 128 (1) | 4 (1)I | 16 (1)I | 16 (1)I | 64 (1)I | 2 (0.50)S | 64(0.50)S | 3/9 (27.27 %) | ||
| B | MIC/2 | 8 (˂0.03)S | ˂1 (˂0.06)S | 32 (0.25)S | ˂1 (˂0.25)S | <1 (<0.06)S | 8 (0.50)S | 16 (0.25)S | 4 (1)I | 32(0.25)S | 8/9 (88.89 %) | |
| MIC/5 | 128 (˂0.50)S | 8 (0.50)S | 128 (1)I | 2 (0.50)S | 4 (0.25)S | 16 (1)I | 32 (0.50)S | <1 (<0.25)S | 64(0.50)S | 6/9 (66.67 %) | ||
| AMP | 0 | - | - | - | - | - | - | - | - | - | ||
| L | MIC/2 | 128 (˂0.50)S | 128 (˂1)S | -(≥1) | -(≥1) | -(≥1) | - (≥1) | -(≥1) | -(≥1) | -(≥1) | 2/9 (22.22 %) | |
| MIC/5 | 256 (˂1)S | -(≥1) | -(≥1) | -(≥1) | -(≥1) | - (≥1) | -(≥1) | 128 (<0.50)S | -(≥1) | 2/9 (22.22 %) | ||
| B | MIC/2 | 128 (˂0.50)S | -(≥1) | 256 (˂1)S | -(≥1) | 256 (<1)S | 256 (<1)S | -(≥1) | -(≥1) | 256(<1)S | 5/9 (55.55 %) | |
| MIC/5 | 256 (˂1)S | -(≥1) | -(≥1) | -(≥1) | -(≥1) | - (≥1) | -(≥1) | 256 (˂1)S | -(>1) | 2/9 (22.22 %) | ||
| CHL | 0 | 32 | 64 | 64 | - | 8 | 64 | 128 | 16 | 128 | ||
| L | MIC/2 | 16 (0.50)S | 32 (0.50)S | 64 (1)I | 128 (˂0.50)S | 16 (2)A | 8 (0.13)S | 64 (0.50)S | 2 (0.13) | 64(0.50)S | 6/9 (66.67 %) | |
| MIC/5 | 32 (1)I | 64 (1)I | 64 (1)I | -(˃1) | 16 (2)A | 16 (0.25)S | 64 (0.50)S | 8 (0.50)S | 128(1)I | 3/9 (27.27 %) | ||
| B | MIC/2 | 32 (1)I | 32 (0.50)S | 32 (0.50)S | 256 (˂1)S | 2 (0.25)S | 16 (0.25)S | 64 (0.50)S | 4 (0.25)S | 64(0.50)S | 8/9 (88.89 %) | |
| MIC/5 | 32 (1)I | 32 (0.50)S | 64 (1)I | -(˃1) | 2 (0.25)S | 32 (0.50)S | 128 (1)I | 8 (0.50)S | 64(0.50)S | 5/9 (55.55 %) | ||
aAntibotics [TET tetracycline, CIP ciprofloxacin, KAN kanamycin, CHL chloramphenicol, AMP ampicillin]. bBacterial strains: Escherichia coli [AG102, AG100Atet], Pseudomonas aeruginosa [PA01, PA124], Enterobacter aerogenes [CM64, EA27], Enterobacter cloacae [BM67], Klebsiella pneumoniae [KP55], Providencia stuartii [NAE16]. cPBSS: percentage of bacteria strain on which synergism has been observed
(): fold increase in MIC values of the antibiotics after association with plants extract; S synergy, I indifference, na not applicable, B bart extract, L leaves extract, FIC fractional inhibitory concentration, (−): >256 μg/mL; 0: no extract (only antibiotic tested)