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. 2015 Sep 17;1(8):e28. doi: 10.1097/TXD.0000000000000539

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Copyright © 2015 The Authors. Transplantation Direct. Published by Wolters Kluwer Health, Inc.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.

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Skin transplantation procedure in GF mice. A, Zone 1 with reusable cold pack submerged in a beaker of gluteraldehyde secured with rubber coated lead weight. B, Sterilization cylinder with transport sleeve positioned to introduce GF mice and sterile caging material from flexible film isolator into zone 1 of BSC. C, Sterile cage containing a sterile wide mouth Nalgene bottle introduced into zone 1/zone 2 used to transport postoperative mice from BSC to isolator. D, Surgeon wearing magnification visor preparing surgical site at left edge of zone 2. E, Placement of skin graft on dorsal thorax of recipient GF mouse. F, Bandaged, anesthetized, postoperative recipient mouse. G, Fiber optic light positioned within the BSC in zone 3 with sterile mylar film over light source attached to sterile orthopedic stockinette, covered with sterile aluminum foil and secured with sterile umbilical tape to sterile metal stand.