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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Immunol. 2015 Oct 26;195(11):5404–5414. doi: 10.4049/jimmunol.1402933

Fig. 5. miR-26b activates NF-κB signaling.

Fig. 5

(A) Raw 264.7 cells were co-transfected with a NF-κB luciferase-reporter construct and miR-26b or miR-Con. After 24 h culture, cells were stimulated with or without LPS (CON). Blank Control (BC): Raw 264.7 cells transfected with only NF-κB luciferase-reporter construct. The firefly luciferase activity was normalized to Renilla luciferase activity. Results are expressed as fold change over blank control (BC) without LPS stimulation. Results are expressed as means ± SE. **P<0.05 vs. miR-Con with LPS, *P<0.05 vs. CON (n=3 cell preparation). (B) Western blot analysis of phosphorylated Akt, Ikk, IκBα and p65 in lysates from bAMs infected with miR-26b or control lentivirus (VC) at a MOI of 50 for 16 h, and then treated for 0 – 60 min (above lanes) with LPS. Blank control (BC): bAMs without virus infection.