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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Neuropharmacology. 2015 Aug 13;99:471–480. doi: 10.1016/j.neuropharm.2015.08.018

Figure 1. Co-expression of Neto1 or Neto2 with GluK1 alters glutamate sensitivity.

Figure 1

A. Whole-cell current traces from HEK-293T cells transiently transfected with GluK1 alone, or in combination with either human Neto1 or rat Neto2. Cells were voltage-clamped at −70 mV in the whole-cell configuration. Glutamate was applied for 5 sec (solid bar) at the concentration indicated.

B. Glutamate concentration-response relationships were constructed by normalizing the peak amplitude of the current response to the maximum response to saturating agonist levels for each cell. Symbols represent mean ± SEM. Averaged data was fit with a sigmoidal curve (solid or dashed lines). The glutamate EC50s for the fits shown were 124.1 μM (GluK1, n=5), 4.3 μM (+Neto1, n=7) and 11.7 μM (+Neto2, n=6).

C. Representative whole-cell current traces from cells transfected with GluK1 and mouse Neto1. Responses obtained as described in A.