Figure 2. Effect of Neto1 and Neto2 on macroscopic desensitization kinetics of GluK1 homomers.

A. Outside-out patches were excised from HEK-293T cells transiently transfected as indicated. 10 mM glutamate (100 msec) or 10 μM glutamate (1 sec) was applied (solid line) to patches voltage-clamped at −70 mV. Traces shown are averages of 3-5 applications to the same patch. Inset for GluK1+Neto1 response to 10 mM glutamate shows rebound current following agonist removal.

B. Cells were transiently transfected with GluK1 and Neto2. Responses from outside-out patches were obtained with 1 sec applications of glutamate at the concentration indicated. All traces shown were obtained from different patches. The currents displayed are normalized to the same relative peak amplitude to facilitate comparison of the decay properties.

C. Paired applications of 10 mM glutamate (100 msec) were given 1 sec apart to outside-out patches voltage-clamped at −70 mV. Cells were transfected as indicated.

D. The % recovery was calculated by dividing the peak amplitude of the second response by that of the first and multiplying by 100. To normalize the effect of the non-desensitized receptors as represented by the residual current, the amplitude of the current at the end of the first 100 msec application was subtracted from both responses. *** (p≤0.001) represents a significant difference from GluK1 receptors without Neto.