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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Diagn Ther. 2015 Dec;19(6):409–417. doi: 10.1007/s40291-015-0170-3

Fig 1. TD-PCR.

Fig 1

In contrast to standard PCR, primers of TD-PCR are oriented away from each other so that a product forms only when 2 sets of primers are juxtaposed because of an ITD (a). The primer span is defined as the length between the 3’ ends of the reverse and forward primers. The sequences of forward and reverse primers are complementary to each other to reduce primer span (c, primer pair TD7). Mismatched nucleotides (capitalized) were introduced at the 5’end and the middle portion of the primers. Seven primer pairs are distributed across exon 14 and intron 14 (c). The primer span of each pair is in parentheses.