. Author manuscript; available in PMC: 2016 Dec 1.

Published in final edited form as: Mol Diagn Ther. 2015 Dec;19(6):409–417. doi: 10.1007/s40291-015-0170-3

Table 1.

Detection of MRD in remission samples by TD-PCR

			TD-PCR^b
Specimen	Interval before relapse	50 ng	250 ng	250 ng
1	6 months	0/2	2/2
2	4 months	0/2	0/2	1/2
3A	4 months	2/2
3B	5 months	2/2
4A	14 months	0/2	1/2
4B^a	5 months	2/2
4C^a	4 months	0/2	1/2
4D^a	1 month	2/2
5	3 months	1/2
6	9 months	2/2
7A	5 months	2/2
7B	2 months	2/2

suspicious for early relapse by flow cytometry.

TD-PCR was conducted with a sequential increase of DNA input for negative specimens until all 12 marrows had MRD detected (50 ng, 250 ng, and additional 250 ng DNA in duplicates) using primer pair TD3 or TD7. The numerator represents the replicate number of positive TD-PCR assays, and the denominator represents the total number of assays.