Table 2.

Cytisine-sensitive [¹²⁵I]-Epibatidine Binding (fmol/mg wet weight)

Plot code	Brain Region	Saline N=6 mice	1.0 mg/kg/hr Nicotine N=6 mice	0.12 mg/kg/hr Varenicline N=5 mice	Nicotine plus Varenicline N=6 mice	Main effect of Nicotine	Main effect of Varenicline	Nicotine x Varenicline interaction
A	Frontal Cortex	3.10±0.13	*4.50±0.37	4.13±0.12	*4.76±0.36	F1,20=10.61	F1,20=4.49	F1,20=1.63
B	Orbital Cortex	5.26±0.51	7.19±0.62	6.70±0.34	7.87±0.45	F1,20=9.25	F1,20=4.35	F1,20=0.55
C	Cingulate Cortex	7.57±0.79	9.48±1.00	9.42±0.72	10.48±1.17	F1,20=2.24	F1,20=2.05	F1,20=0.18
D	Cortex, Outer	3.43±0.17	*5.01±0.48	*5.45±0.27	*5.31±0.26	F1,20=5.01	F1,20=12.94	F1,20=7.20
E	Cortex, Inner	6.02±0.33	7.89±0.58	*9.28±0.42	*9.35±0.50	F1,20=4.01	F1,20=24.03	F1,20=3.50
F	Retrosplenial Cortex	10.23±0.69	12.24±0.79	*14.30±1.19	13.60±1.19	F1,20=2.55	F1,20=7.20	F1,20=1.79
G	Hippocampus	3.38±0.20	4.36±0.30	*4.98±0.17	*5.55±0.22	F1,20=11.03	F1,20=35.70	F1,20=0.71
H	Subiculum	12.03±0.41	*17.37±1.64	*16.79±1.00	*18.44±0.96	F1,20=6.57	F1,20=6.03	F1,20=1.61
I	Septum	6.23±0.30	7.04±0.36	7.63±0.44	*8.74±0.72	F1,20=3.39	F1,20=8.73	F1,20=0.83
J	Olfactory Tubercle	4.28±0.28	*6.04±0.43	*6.86±0.66	*7.02±0.45	F1,20=3.61	F1,20=17.28	F1,20=3.31
K	Nucleus Accumbens	4.86±0.26	*6.40±0.35	*6.37±0.55	*7.23±0.41	F1,20=12.72	F1,20=14.88	F1,20=0.32
L	Striatum	6.71±0.41	8.15±0.60	7.93±0.70	9.32±0.55	F1,20=6.71	F1,20=5.91	F1,20=0.04
M	Substantia nigra pars compacta	11.30±1.19	14.42±2.45	14.41±1.28	16.32±1.04	F1,20=2.52	F1,20=2.04	F1,20=0.19
N	Substantia nigra, pars reticulate	10.24±1.74	13.75±2.24	10.89±1.76	14.12±1.31	F1,20=2.27	F1,20=0.28	F1,20=0.04
O	Ventral Tegmental Area	15.74±1.75	20.75±3.99	19.51±1.87	23.06±0.76	F1,20=3.24	F1,20=1.45	F1,20=0.12
P	Anterodorsal thalamic nucleus	28.42±1.56	27.55±1.94	28.00±2.55	33.08±2.21.	F1,20=1.00	F1,20=1.48	F1,20=2.00
Q	Laterodorsal thalamic nucleus	21.98±1.24	23.73±1.19	24.14±1.82	26.34±1.15	F1,20=2.18	F1,20=2.19	F1,20=0.03
R	Mediodorsal thalamic nucleus	22.63±1.42	24.23±1.39	23.47±1.79	26.46±1.27	F1,20=2.43	F1,20=1.10	F1,20=0.22
S	Ventrolateral thalamic nucleus	17.53±0.94	20.00±3.71	22.07±2.00	22.62±1.11	F1,20=1.19	F1,20=6.68	F1,20=0.48
T	Hypothalamus	5.70±0.48	7.53±0.44	*8.78±0.80	*10.32±0.30	F1,20=11.34	F1,20=34.41	F1,20=0.08
U	Medial Habenula	63.62±11.60	60.77±13.20	38.89±14.24	70.73±15.22	F1,20=3.24	F1,20=0.23	F1,20=2.62
V	Fasiculus Retroflexus	22.86±1.70	29.64±6.79	26.48±6.82	33.30±4.44	F1,20=1.20	F1,20=1.09	F1,20=0.17
W	Interpeduncular nucleus	49.45±13.65	68.91±19.16	30.23±19.84	62.01±12.20	F1,20=2.17	F1,20=0.37	F1,20=0.01
X	Optic tracts	4.21±0.43	4.42±0.70	5.32±1.41	5.49±0.44	F1,20=0.48	F1,20=6.21	F1,20=0.05
Y	Dorsolateral geniculate nucleus	21.83±2.83	24.77±3.32	22.50±2.47	24.98±1.52	F1,20=1.74	F1,20=0.00	F1,20=0.07
Z	Pregeniculate nucleus	14.26±1.34	14.90±2.50	14.04±1.74	16.77±1.35	F1,20=0.58	F1,20=0.11	F1,20=0.10
a	Zona Incerta	10.37±0.30	13.37±1.15	*15.63±1.35	*16.18±0.96	F1,20=3.14	F1,20=16.30	F1,20=1.49
b	Olivary Pretectal Nucleus	10.53±1.06	*13.64±2.85	*14.78±1.70	*19.82±1.06	F1,20=4.73	F1,20=3.85	F1,20=1.90
c	Superior Colliculus, superficial gray	11.99±1.19	12.65±2.09	11.56±2.45	15.36±1.39	F1,20=2.19	F1,20=2.85	F1,20=0.20
d	Superior Colliculus, optic nerve layer	17.65±1.00	20.83±1.93	21.07±2.02	23.43±1.62	F1,20=3.10	F1,20=3.63	F1,20=0.05
e	Medial Geniculate Nucleus	16.21±1.08	18.60±2.53	18.91±1.61	20.91±0.96	F1,20=1.64	F1,20=1.99	F1,20=0.02
f	Inferior Colliculus, Dorsal Cortex Anterior	7.30±0.50	*9.65±0.57	*11.42±0.78	*12.75±0.40	F1,17=10.20	F1,17=39.06	F1,17=0.78
g	Inferior Colliculus, Central Nucleus	4.09±0.39	*6.09±0.67	*5.83±0.36	*6.51±0.28	F1,19=7.78	F1,19=5.05	F1,19=1.89
h	Inferior Colliculus, External Cortex	3.36±0.47	4.82±1.00	*5.02±0.55	*6.82±1.07	F1,15=3.23	F1,15=9.47	F1,15=0.32
i	Inferior Colliculus, Dorsal Cortex Posterior	4.48±0.72	*9.04±1.76	*10.63±2.04	*8.10±1.41	F1,13=0.67	F1,13=7.61	F1,13=5.50
j	Anterior Olfactory Area	0.98±0.13	0.84±0.16	1.88±0.76	1.11±0.18	F1,20=0.03	F1,20=4.56	F1,20=1.46
k	Accessory Olfactory Bulb	0.83±2.10	3.48±2.08	1.98±1.45	4.47±2.21	F1,20=3.26	F1,20=0.13	F1,20=0.02
l	Periaquiductal Gray	8.72±0.51	*12.37±1.64	*12.08±1.04	*13.21±0.85	F1,19=4.50	F1,19=3.46	F1,19=1.24
m	Deep Mesencephalic Area	9.72±0.61	*13.42±1.66	*13.46±1.01	*15.78±0.96	F1,19=6.37	F1,19=6.53	F1,19=0.34
n	Pontine Nucleus	10.78±1.19	12.44±1.44	14.33±2.37	16.69±2.31	F1,18=0.42	F1,12=2.81	F1,12=0.07
o	Dorsal Tegmental Nucleus	9.13±1.42	11.39±0.77	*14.10±2.02	*14.80±1.58	F1,12=0.50	F1,12=10.67	F1,12=1.63
p	Hindbrain	6.29±1.32	8.27±0.72	11.04±2.02	10.03±1.13	F1,12=3.07	F1,12=3.47	F1,12=0.92
q	Cerebellum	1.05±0.07	1.80±0.29	1.49±0.30	1.80±0.33	F1,12=2.64	F1,12=0.44	F1,12=0.46