(A) IgM was measured by ELISA in the supernatants of B-lymphocytes from NTC, treated with different β-glucan preparations or CpG as indicated for a total of 5 days. (B) 3H-Thymidine incorporation was assessed in NTC, stimulated cells with different β-glucan preparations, CpG or Immunoglobulin cocktail control. Shown are data from a representative experimental run, typical of at least three separate experimental preparations, performed with different B-cell preparations. *p<0.0001 when compared with the NTC.