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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Immunol. 2015 Oct 30;195(11):5318–5326. doi: 10.4049/jimmunol.1500559

Figure 6. MAPKs (ERK and JNK) and the transcription factors AP-1 and NF-κB participate in IL-8 secretion.

Figure 6

(A) Immunoblot analysis of ERK and JNK (phosphorylated and total) in NTC and stimulated cells with Curdlan or Zymosan as indicated; (B) for some conditions cells were pretreated with PD98059 (ERK inhibitor) or JNK inhibitor II as indicated. (C) IL-8 concentration was measured by ELISA in the cell supernatant of NTC and after Curdlan stimulation. Cells were pretreated with PD98059 and JNK inhibitor II or (D) BAY11 (NF-κB inhibitor), SR11 (AP-1 inhibitor) as indicated. DMSO was used as the vehicle control. Shown are data from a representative experimental run, typical of at least three separate experimental preparations, performed with different B-cell preparations. *p<0.0001 when compared with DMSO control.