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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Immunol. 2015 Oct 30;195(11):5318–5326. doi: 10.4049/jimmunol.1500559

Figure 7. Conditioned media from β-Glucan-activated B-lymphocytes induced neutrophil chemotaxis and is partially mediated by IL-8.

Figure 7

Transwell chemotaxis assay of peripheral neutrophils towards different concentrations of (A) fMLF peptide (positive control) or (B) towards conditioned media form β-glucan treated B-lymphocytes for the indicated periods of time. Shown are data from a representative experimental run, typical of at least three separate experimental preparations, performed with different B-cell preparations. *p<0.05 and **p<0.01 when compared with NTC. Transwell chemotaxis assay of peripheral neutrophils towards different concentrations of recombinant IL-8 (C) or conditioned media (D) from β-glucan treated B cells in the presence or absence of anti-IL-8 or isotype control. *p<0.001 and **p<0.0001. Shown are data from a representative experimental run, typical of at least three separate experiments performed with different B cell preparations.