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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Methods. 2015 May 26;90:68–75. doi: 10.1016/j.ymeth.2015.05.021

Figure 3. Titration of glycoproteins.

Figure 3

(A). Cell sSurface expression (CELISA). B78 cells were transfected with varying concentrations of DNA corresponding to one viral glycoprotein (gB, gD, or gH/gL) while the other three were maintained at 125ng along with one of the DSP plasmids. Data were normalized by setting the absorbance value for cells transfected with 125ng of each glycoprotein (filled bars) to be 100%. (B). Rates of fusion obtained from luciferase activity. B78 cells were transfected the same way as for panel A, and fusion was triggered with C10 cells carrying the second DSP half as shown in Figure 2. Rates were determined by taking the luminescence units (normalized by considering the signal at 7h to be 100%) between 3 and 7h and calculating the slope of that line between these time points. Data from 3 independent experiments were averaged; R2 value was equal to or higher than 0.96. (Copyright © American Society for Microbiology, Journal of Virology, 2013 Nov; 87(21): 11332–11345. DOI: 10.1128/JVI.01700-13)