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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Methods. 2015 May 26;90:68–75. doi: 10.1016/j.ymeth.2015.05.021

Figure 5. Use of soluble gD(gD306t) to trigger fusion between receptor bearing target cells and effector cells bearing gB and gH/gL.

Figure 5

(A) Absolute fusion rates are similar when gD is expressed from a plasmid (A) or delivered as a soluble protein (B). (BC).Dose-dependence of fusion on gD306t concentration. Curves show correlation between fusion levels and different concentrations of gD306t. Concentration ranging between 500 μg/ml and 30μg/ml gave the same signal output as 250μg/ml. (CD).Determining gD306t concentration that yields 50% of maximum fusion level. Data from (C) are plotted as levels of fusion 6 hours post co-cultivation.