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. 2015 Oct 6;156(12):4411–4425. doi: 10.1210/en.2015-1556

Figure 1.

Figure 1.

Shedding of ACE2 in insulinoma cells. The 832/13 cells were cotransfected with 0.5 μg ACE2 expression plasmids per well and the indicated amounts of the ADAM17 expression plasmid pAd17 or pcDNA3.1(−) in two experiments, with each treatment given to duplicate wells. A–C, Western blots of cellular ACE2, ACE2 released into the cell culture medium, and cellular ADAM17, respectively. D–F, Densitometric analyses of band intensities observed in the Western blots for cellular ACE2, shed ACE2, and cellular ADAM17, respectively. Bands were normalized relative to the average intensity of bands of interest on each blot, which was set at a value of 100. *,***, P < .05, P < .001 vs 0 μg pAd17; ###, P < .001 vs 0.5 μg pAd17 for post hoc contrasts of means after an ANOVA.