ACE2 and ADAM17 in mouse pancreatic islet cells. A, Dispersed islet cells were assessed for red tdTomato fluorescence. The lower panel shows a cell subpopulation with high red fluorescence that is sorted from the non-red cells. B, Quantitative RT-PCR was performed to measure ACE2, ADAM17, insulin 2, glucagon, and somatostatin mRNA for sorted red and non-red islet cells from four female Tom+/−, Ins2-Cre+/− mice. C, ACE2 activity was determined for sorted red and non-red islet cells from two female and one male Tom+/−, Ins2-Cre+/− mice. *, **, P < .05, P < .01 vs non-red cells as determined by paired t tests.