Skip to main content
. 2015 Oct 6;156(12):4411–4425. doi: 10.1210/en.2015-1556

Figure 8.

Figure 8.

Effects of inhibiting ADAM17 activity on ACE2 expression. 832/13 cells were transfected in four experiments with 100 ng/well mACE2/pcDNA3.1 and cotransfected with 2 μg/well of pAd17 or pAd17E406A. After transfection, cells were incubated in the absence or presence of 1 μM DPC-333. We determined the cellular ACE2 activity (A) and calculated the ratio of total shed ACE2 activity to total cellular ACE2 (B). *, **, ***, P < .05, P < .01, P < .001 vs no DPC-333; ###, P < .001 vs pAd17 in the absence of DPC-333 for post hoc contrasts of means after an ANOVA. Pancreatic islets were isolated from C57BL/6 mice aged 4–12 months. Pools of islets from four mice each were generated for a total of three pools from male mice and two pools from female mice. Aliquots of the islets were incubated for 24 hours in RPMI 1640 medium without serum. We determined the cellular ACE2 activity (C) and calculated the ratio of total shed ACE2 activity to total cellular ACE2 (D). There were no significant differences for post hoc contrasts of means in the absence and presence of DPC-333 after an ANOVA, but the main effect for sex was significant at P < .001 for cellular ACE2 activity.