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. 2015 Sep 21;156(12):4731–4740. doi: 10.1210/en.2015-1464

Figure 1.

Figure 1.

IGF-1R expression in TSHR+/+ and TSHR−/− female mouse thyroid and lung fibroblasts. A and B, Thyroid tissues were harvested from mice and RNA immediately extracted as described in Materials and Methods. Lung fibroblasts were allowed to proliferate to confluence, harvested, processed, and cellular RNA extracted as described in Materials and Methods. Each sample was reversed transcribed and subjected to real-time PCR for TSHR (A) or IGF-1R (B) mRNA. Data are expressed as the mean ± SD of three replicates. Data from lung fibroblasts represent those from five mice in each group. ND, not detected. C and D, Flow cytometric analysis of cell surface IGF-1R and intracellular collagen 1 in TSHR+/+ and TSHR−/− lung fibroblasts. C, Aggregate results, expressed as MFI from five fibroblast strains, each from a separate animal. Data are expressed as the mean ± SD. D, Individual examples of cytometric gating strategies used in analyses the results from which comprise panel C.